Summary
Type: SPE
Application #: 304010
Catalog#: 730300 - SPE Cartridges, Chromabond, C18 Hydra, 6ml, PP, 1000mg 30/pk
Detection:
Conditions: Column conditioning: 2 x 6 mL dichloromethane – acetone (1:1, v/v) under vacuum, then 6 mL methanol without vacuum (3 min); Do not let run the column dry.
Sample aspiration: 500 mL of pretreated sample is passed through the column by vacuum.
Elution: 6 mL dichloromethane – acetone (80:20, v/v), then 6 mL dichloromethane – acetone (20:80, v/v)
Evaporation: to final volume of 0.05 mL (gentle stream of nitrogen)
Authors: Boti, V.I. et al.
Source: University of Ioannina, Greece